WNT4 anticorps (Internal Region)

N° du produit ABIN1049477

Détail du produit anti-WNT4 anticorps

Antigène: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cheval, Singe, Porc, Chien, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WNT4 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human WNT4. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except WNT5A (71 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (100%) Opossum (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 14 amino acid peptide from internal region of human WNT4. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Pig (100%), Opossum (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur WNT4

Antigène: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Autre désignation: WNT4 (WNT4 Produits)

Synonymes: anticorps CG4698, anticorps DWnt-4, anticorps DWnt4, anticorps Dm DWnt4, anticorps Dmel\\CG4698, anticorps Dwnt4, anticorps Wnt, anticorps Wnt-4, anticorps anon-EST:Liang-2.4, anticorps clone 2.4, anticorps wnt-4, anticorps wnt4, anticorps SERKAL, anticorps WNT-4, anticorps Xwnt4, anticorps xwnt-4, anticorps zgc:136737, anticorps Wnt family member 4, anticorps Wnt oncogene analog 4, anticorps wingless-type MMTV integration site family, member 4, anticorps wingless-type MMTV integration site family member 4 S homeolog, anticorps wingless-type MMTV integration site family, member 4a, anticorps WNT4, anticorps Wnt4, anticorps wnt4.S, anticorps wnt4a

Sujet: Name/Gene ID: WNT4
Family: WNT

Synonyms: WNT4, WNT-4, Protein Wnt-4, SERKAL

ID gène: 54361

Pathways: Signalisation WNT, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cell-Cell Junction Organization, Tube Formation