POLI anticorps (Middle Region, N-Term)

N° du produit ABIN1107000

Détail du produit anti-POLI anticorps

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Épitope: Middle Region, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLI est non-conjugé

Application: Western Blotting (WB)

Séquence: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Immunoaffinity column

Immunogène: Synthetic peptide directed towards the N-terminal to middle region of human POLI

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Add 50 μL of distilled water to a final concentration of 1 mg/mL.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized at 2-8 °C for one month or at -20 °C long term. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or in aliquots at -20 °C long term.

Détails sur POLI

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Autre désignation: DNA Polymerase iota (POLI Produits)

Synonymes: anticorps poli, anticorps wu:fc27h11, anticorps zgc:110185, anticorps GB12093, anticorps NV16187, anticorps RAD30B, anticorps RAD3OB, anticorps Rad30b, anticorps DNA polymerase iota, anticorps polymerase (DNA directed) iota, anticorps polymerase (DNA directed), iota, anticorps POLI, anticorps poli, anticorps LOC551219, anticorps AFUA_4G11140, anticorps LOC5568276, anticorps Pol-iota, anticorps Poli

Sujet: DNA polymerase iota (POLI) is an error-prone DNA polymerase specifically involved in DNA repair. POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. POLI favors Hoogsteen base-pairing in the active site. POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. POLI may play a role in hypermutation of immunogobulin genes. POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.Synonyms: Eta2, POLI, RAD30 homolog B, RAD30B

Poids moléculaire: POLI: 83 kDa

ID gène: 11201

NCBI Accession: NM_007195

Pathways: Réparation de l'ADN