CD11c anticorps (APC)

N° du produit ABIN1112431

Détail du produit anti-CD11c anticorps (APC)

Antigène: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11c est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Monoclonal Hamster Anti-Mouse CD11c APC is recommended for use in flow cytometry for identification of the Mouse CD11c.

Immunogène: Murine spleen dendritic cells

Clone: N418

Isotype: IgG

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. Recommeded Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometry analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal tless or equal to 0.25 µg per 10^6 cells in 100 µL volume or 100 µL of whole bloodIt is recommended that the reagent be titrated for optimal performance for each application.

Commentaires:

Allophycocianin (Abs/ Em: 651/ 662 nm).

Préparation des réactifs:

1. Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add antibody and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided Phosphate-buffered solution, pH 7.2, containing 0.09% Sodium azide and 0.2% (w/v) BSA (Sigma).

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Références pour anticorps anti-CD11c (APC) (ABIN1112431)

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, (1995) (PubMed).

Détails sur CD11c

Antigène: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Autre désignation: CD11c (ITGAX Produits)

Synonymes: anticorps CD11C, anticorps SLEB6, anticorps RGD1561123, anticorps AI449405, anticorps Cd11c, anticorps Cr4, anticorps N418, anticorps integrin subunit alpha X, anticorps integrin alpha X, anticorps ITGAX, anticorps Itgax

Sujet: CD11c is a 150 kDa glycoprotein, also known as alphaX integrin, CR4, or p150. CD11c forms a alphaXbeta2heterodimer with beta2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The alphaXbeta2 integrin plays an important role in cell-cell contact by binding its ligands, iC3b, fibrinogen, and CD54.

Pathways: Système du Complément, Activated T Cell Proliferation, Integrin Complex