CD45 anticorps (APC)

N° du produit ABIN3044642

Détail du produit anti-CD45 anticorps (APC)

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Monoclonal Rat Anti-Mouse CD45R-APC is recommended for use in flow cytometry for identification of the Mouse CD45R molecule, an epitope on the extracellular domain of CD45 which is expressed on B lymphoid cells.

Immunogène: Mouse pre-B tumour cells

Clone: RA3-6B2

Isotype: IgG2a

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal tless or equal to 0.25 µg per 10^6 cells in 100 µL volume.

Commentaires:

Allophycocyanin (APC). Abs/Em: 651/662 nm.

Préparation des réactifs:

1. Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add CD45R APC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing PBS, 1% BSA and 0.09 % Sodium azide, pH 7,4.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Détails sur CD45

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Autre désignation: CD45R (PTPRC Produits)

Synonymes: anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc

Sujet: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45R is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45R.

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling