TNFRSF1A anticorps (PE)

N° du produit ABIN114262

Détail du produit anti-TNFRSF1A anticorps (PE)

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TNFRSF1A est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: HM104

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur TNFRSF1A

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Autre désignation: CD120a / TNFR1 (TNFRSF1A Produits)

Synonymes: anticorps CD120a, anticorps FPF, anticorps MS5, anticorps TBP1, anticorps TNF-R, anticorps TNF-R-I, anticorps TNF-R55, anticorps TNFAR, anticorps TNFR1, anticorps TNFR1-d2, anticorps TNFR55, anticorps TNFR60, anticorps p55, anticorps p55-R, anticorps p60, anticorps TNF-R1, anticorps TNF-alphaR1, anticorps TNFRI, anticorps TNFRp55, anticorps TNFalpha-R1, anticorps Tnfr-2, anticorps Tnfr1, anticorps fpf, anticorps tbp1, anticorps p55-r, anticorps tnf-r, anticorps tnfar, anticorps tnfr1, anticorps cd120a, anticorps tnfr55, anticorps tnfr60, anticorps xtnfr1, anticorps wu:fj65b10, anticorps zgc:85914, anticorps TNFR I, anticorps TNFRSF1A, anticorps TNFA, anticorps cTNF, anticorps TNF receptor superfamily member 1A, anticorps tumor necrosis factor receptor superfamily, member 1a, anticorps TNF receptor superfamily member 1A S homeolog, anticorps tumor necrosis factor, anticorps TNFRSF1A, anticorps Tnfrsf1a, anticorps tnfrsf1a.S, anticorps tnfrsf1a, anticorps TNF

Sujet: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28 % sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade. TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60

ID gène: 10090

UniProt: P25118

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway