CD45 anticorps (PE)

N° du produit ABIN114377

Détail du produit anti-CD45 anticorps (PE)

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Specificité: This antibody recognizes a monomorphic determinant of the rat leukocyte common antigen (CD45) (1). The antigen recognized is a heavily glycosylated membrane glycoprotein of molecular weight 170,000 kDa on thymocytes but molecular weight 170,000-220,000 kDa on other leukocytes.

Purification: Protein G Chromatography.

Immunogène: Lymph node glycoproteins and cells.

Clone: OX-30

Isotype: IgG2a

Information d'application

Indications d'application: Suitable for use in Flow cytometry (See Protocol).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, 0. 5 µg-1. 0 µg of ABIN114377 or ABIN114377X per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes arelight sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: (Figure 1)Rat Strain: WistarCell Concentration: 1x10e6 cells per test. Antibody Concentration Used: 0. 5 µg/10e6 cells. Isotypic Control: PE Mouse IgG2a. Cell-Source Percentage of cells stained above control: Thymus: 99. 8%Spleen: 97. 1%Lymph Node: 98. 9%Strain Distribution: Strains Tested: Wistar, Buffalo, Brown Norway, Fischer 344Positive: Wistar, Buffalo, Brown Norway, Fischer 344Negative: none

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS with 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions.

Détails sur CD45

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Autre désignation: CD45 / LCA (PTPRC Produits)

Synonymes: anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc

Sujet: The leukocyte common antigen (L-CA) is a major glycoprotein of haematopoietic cells but is not found on other tissues or erythroid cells. It is present on greater than 95 % of thymocytes, bone marrow cells and thoracic duct lymphocytes. This molecule carries much of the carbohydrate of thymocytes and shows interesting heterogeneity amongst T lymphocytes and B lymphocytes. (2,3).Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

ID gène: 24699

NCBI Accession: NP_001103357

UniProt: P04157

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling