AKT3 anticorps

N° du produit ABIN1892292

Détail du produit anti-AKT3 anticorps

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100 % identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.

Purification: Protein A purified

Stérilité: Sterile filtered

Immunogène: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.

Type of Immunogen: Synthetic peptide

Clone: 9A5-H9-G7

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA (1:2000 - 1:10000), IHC (20 μg/mL), WB (1:500 - 1:2000)

Usage: Anti-AKT3 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, sterile filtered

Conseil sur la manipulation: avoid freeze thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Détails sur AKT3

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Autre désignation: AKT3 (AKT3 Produits)

Synonymes: anticorps MPPH, anticorps PKB-GAMMA, anticorps PKBG, anticorps PRKBG, anticorps RAC-PK-gamma, anticorps RAC-gamma, anticorps STK-2, anticorps AI851531, anticorps D930002M15Rik, anticorps Nmf350, anticorps Pkbg, anticorps AKT serine/threonine kinase 3, anticorps thymoma viral proto-oncogene 3, anticorps v-akt murine thymoma viral oncogene homolog 3b, anticorps AKT3, anticorps Akt3, anticorps akt3b

Sujet: Name/Gene ID: AKT3
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2

ID gène: 10000

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Signalisation TLR, Hepatitis C, VEGF Signaling