GSK3 alpha anticorps (pSer21)

N° du produit ABIN196620

Cet anticorps anti-GSK3 alpha Polyclonal Lapin (ABIN196620) détecte spécifiquement GSK3 alpha dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour GSK3 alpha anticorps (pSer21) (ABIN196620)

Antigène: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GSK3 alpha est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GSK3 alpha anticorps

Épitope: pSer21

Specificité: GSK3alpha (phospho-Ser21) antibody detects endogenous levels of GSK3alpha only when phosphorylated at serine 21.

Purification: Immunoaffinity chromatography

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human GSK3alpha around the phosphorylation site of serine 21 (T-S-SP-F-A).

Information d'application

Indications d'application: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02 % Sodium Azide as preservative and 50 % glycerol as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur GSK3 alpha

Antigène: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Autre désignation: GSK3 alpha

Sujet: Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily, GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97 % sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

ID gène: 2931

NCBI Accession: NP_063937

UniProt: P49840

Pathways: Signalisation PI3K-Akt, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, L'effet Warburg