CD45 anticorps

N° du produit ABIN1981850

Détail du produit anti-CD45 anticorps

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivité: Humain, Souris, Cat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func)

Specificité: The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

Réactivité croisée (Details): Human, Mouse, Feline (Cat)

Purification: Purified by protein-G affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

niveau d'endotoxine: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogène: Abelson murine leukemia virus-induced pre-B tumor cells

Clone: RA3-6B2

Isotype: IgG2a kappa

Information d'application

Indications d'application: Functional application: Modulation of B cell responses.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze. Do not use after expiration date stamped on vial label.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD45 (ABIN1981850)

Lochhead, Sonderegger, Ma, Brewster, Cornwall, Maylor-Hagen, Miller, Zachary, Weis, Weis: "Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2488-501, (2012) (PubMed).

Montecino-Rodriguez, Leathers, Dorshkind: "Identification of a B-1 B cell-specified progenitor." dans: Nature immunology, Vol. 7, Issue 3, pp. 293-301, (2006) (PubMed).

Rothaeusler, Baumgarth: "Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry." dans: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 69, Issue 4, pp. 249-59, (2006) (PubMed).

Cappione, Pugh-Bernard, Anolik, Sanz: "Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4298-307, (2004) (PubMed).

Tsurui, Nishimura, Hattori, Hirose, Okumura, Shirai: "Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 48, Issue 5, pp. 653-62, (2000) (PubMed).

Rada, Ehrenstein, Neuberger, Milstein: "Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting." dans: Immunity, Vol. 9, Issue 1, pp. 135-41, (1998) (PubMed).

Watanabe, Akaike: "Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells." dans: Scandinavian journal of immunology, Vol. 39, Issue 5, pp. 419-25, (1994) (PubMed).

Détails sur CD45

Antigène: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Autre désignation: CD45R (PTPRC Produits)

Synonymes: anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc

Sujet: CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.,PTPRCR, T200R

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling