For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Fixation with PFA or acetone/methanol is recommended.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Date de péremption
12 months
Kawabe, Nakanishi, Asada, Fukuhara, Morimoto, Takeuchi, Takai: "Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells." dans: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48350-5, (2001) (PubMed).
Sakisaka, Nakanishi, Takahashi, Mandai, Miyahara, Satoh, Takaishi, Takai: "Different behavior of l-afadin and neurabin-II during the formation and destruction of cell-cell adherens junction." dans: Oncogene, Vol. 18, Issue 8, pp. 1609-17, (1999) (PubMed).
Antigène
PVRL2
(Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))
Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.