Cet anticorp Serotonin Receptor 1A est non-conjugé
Application
Western Blotting (WB), ELISA
Purification
HTR1A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
HTR1A antibody was raised against a 14 amino acid synthetic peptide near the internal region of HTR1A (aa 249-262).
HTR1A
Reactivité: Humain, Rat, Souris
WB, ELISA, IHC, IF, IHC (p), ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 37 kDa and 35 kDa in human brain (hippocampus, Substantia nigra) lysates after 0.1 μg/mL antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.