PDK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
PDK1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of PDK1.
PDPK1
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 65-70 kDa band observed in rat heart lysates (calculated MW of 49.2 kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))