Cullin 2 anticorps

N° du produit ABIN265376

Détail du produit anti-Cullin 2 anticorps

Antigène: Cullin 2 (CUL2)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cullin 2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of Cullin-2 protein (region surrounding Ile726).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Cullin 2

Antigène: Cullin 2 (CUL2)

Autre désignation: Cullin-2 (CUL2 Produits)

Synonymes: anticorps cul2, anticorps Cullin-2, anticorps fj48a06, anticorps wu:fj48a06, anticorps cullin-2, anticorps LOC100221330, anticorps 1300003D18Rik, anticorps 4932411N15Rik, anticorps AI327301, anticorps mKIAA4106, anticorps ATCUL2, anticorps F22D16.2, anticorps F22D16_2, anticorps cullin 2, anticorps cullin 2, anticorps cullin-2, anticorps putative cullin-2 (cul-2), anticorps cullin 2 L homeolog, anticorps Cullin-2, anticorps cul2, anticorps CUL2, anticorps PTRG_05231, anticorps Smp_079740, anticorps LOC100550749, anticorps Cul2, anticorps cul2.L, anticorps cul-2

Sujet: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF(Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.Synonyms: CUL-2, CUL2

Poids moléculaire: approx. 82, 84 kDa

ID gène: 8453

NCBI Accession: NP_003582

UniProt: Q13617

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome