CD11b anticorps

N° du produit ABIN2688915

Détail du produit anti-CD11b anticorps

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: The ICRF44 monoclonal antibody reacts with CD11b, the 165- kDa adhesion glycoprotein that associates with the 95- kDa integrin β2 (CD18) to form the CD11b/CD18 complex, also known as Mac-1 or CR3. CD11b is expressed on activated lymphocytes, monocytes, granulocytes, and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a receptor for iC3b, CD54 (ICAM-1), CD102 (ICAM-2) and CD50 (ICAM-3). This antibody significantly inhibits polymorphonuclear leukocyte aggregation in response to fMLP.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human monocytes.

Clone: ICRF44

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistochemistry: The ICRF44 (44) antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was human spleen and tonsil. The antibody stains activated lymphocytes, monocytes, granulocytes and a subset of NK cells. The isotype control recommended for use with this antibody is purified mouse IgG1. For optimal indirect immunohistochemical staining, the ICRF44 (44) antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB detection system. The clone ICRF44 (44) is not recommended for formalin-fixed paraffin embedded sections.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 62.5 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD11b (ABIN2688915)

David, Kacher, Specks, Aviram: "Interaction of proteinase 3 with CD11b/CD18 (beta2 integrin) on the cell membrane of human neutrophils." dans: Journal of leukocyte biology, Vol. 74, Issue 4, pp. 551-7, (2003) (PubMed).

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b/Mac-1 (ITGAM Produits)

Synonymes: anticorps CD11b/CD18, anticorps CR3, anticorps CR3A, anticorps Cd11b, anticorps F730045J24Rik, anticorps Ly-40, anticorps MAC1, anticorps Mac-1, anticorps Mac-1a, anticorps CD11B, anticorps MAC-1, anticorps MAC1A, anticorps MO1A, anticorps SLEB6, anticorps C3bi, anticorps integrin subunit alpha M, anticorps integrin alpha M, anticorps integrin, alpha M (complement component 3 receptor 3 subunit), anticorps integrin alpha-M, anticorps ITGAM, anticorps Itgam, anticorps LOC100351865

Sujet: Synonyms: Mac-1α, integrin αM subunit, CR3 α chain

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation