AKT3 anticorps (AA 119-136)

N° du produit ABIN302246

Détail du produit anti-AKT3 anticorps

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Épitope: AA 119-136

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibodydetects V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3). Family: Protein Kinase Subfamily: RAC/AKT

Attributs du produit: Synonyms: RAC-PK-gamma, Protein kinase B gamma, PKBG, STK-2, RAC-gammaserine/threonine-protein kinase

Purification: Protein G Chromatography

Immunogène: Synthetic peptide corresponding to amino acid residues 119-136 of human Akt3AA Sequence: CSPTSQIDNIGEEEMDASRemarks: GenBank no. gi|4574744|gb|AAD24196. 1|AF135794_1. This sequence is identicalin human, mouse, rat, cow, dog, and chicken.

Clone: 66C1247-1

Isotype: IgG1

Information d'application

Indications d'application: Western blot (1 μg/mL - 2 μg/mL). Immunohistochemistry on paraffin sections (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS, 0.05 % Sodium Azide, 0.05 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 - -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur AKT3

Antigène: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Autre désignation: AKT3 / PKB gamma (AKT3 Produits)

Synonymes: anticorps MPPH, anticorps PKB-GAMMA, anticorps PKBG, anticorps PRKBG, anticorps RAC-PK-gamma, anticorps RAC-gamma, anticorps STK-2, anticorps AI851531, anticorps D930002M15Rik, anticorps Nmf350, anticorps Pkbg, anticorps AKT serine/threonine kinase 3, anticorps thymoma viral proto-oncogene 3, anticorps v-akt murine thymoma viral oncogene homolog 3b, anticorps AKT3, anticorps Akt3, anticorps akt3b

Sujet: Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. Akt/PKB phosphorylation is critical for it activity.Synonyms: PKBG, Protein kinase B gamma, RAC-PK-gamma, RAC-gamma serine/threonine-protein kinase, STK-2

ID gène: 10000

UniProt: Q9Y243

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Signalisation TLR, Hepatitis C, VEGF Signaling