MCAM anticorps
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- Antigène Voir toutes MCAM Anticorps
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MCAM est non-conjugé
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogène
- Recombinant human protein was used as the immunogen for the CD146 antibody.
- Clone
- MUC18-1130
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MCAM Anticorps primaire
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- Indications d'application
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Optimal dilution of the CD146 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the CD146 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Autre désignation
- CD146 (MCAM Produits)
- Synonymes
- anticorps CD146, anticorps MUC18, anticorps Muc18, anticorps 1-gicerin, anticorps AV025631, anticorps CD149, anticorps s-endo, anticorps s-gicerin, anticorps cd146, anticorps gicerin, anticorps id:ibd3512, anticorps mcam, anticorps MCAM, anticorps melanoma cell adhesion molecule, anticorps melanoma cell adhesion molecule b, anticorps MCAM, anticorps Mcam, anticorps mcamb
- Sujet
- The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease.
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