TNF alpha anticorps

N° du produit ABIN3026402

Détail du produit anti-TNF alpha anticorps

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Neutralization (Neut)

Aucune reactivité croisée: Souris

Attributs du produit: This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes HurTNFamediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the TNF-alpha antibody.

Clone: J1D9

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the TNF-alpha antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Functional Activity: Inhibits Tumor Growth, Neutralizes rTNF-alpha Mediated Cytotoxicity, Protects Mice Against Toxicity of rhTNF-alpha (Order SAF formulation)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha (TNF alpha Produits)

Synonymes: anticorps DIF, anticorps TNF-alpha, anticorps TNFA, anticorps TNFSF2, anticorps RATTNF, anticorps Tnfa, anticorps tnf, anticorps TNF-a, anticorps TNFalpha, anticorps Tnfsf1a, anticorps TNFa, anticorps cTNF, anticorps Tnf-alpha, anticorps tnfa-like, anticorps TNF-ALPHA, anticorps dif, anticorps tnfa, anticorps xtnf, anticorps tnfsf2, anticorps tnf-alpha, anticorps Cachectin, anticorps tumor necrosis factor, anticorps tumor necrosis factor b (TNF superfamily, member 2), anticorps tumor necrosis factor alpha, anticorps tumor necrosis factor a (TNF superfamily, member 2), anticorps TNF, anticorps Tnf, anticorps tnf, anticorps tnfb, anticorps tnf-alpha, anticorps LOC103694380, anticorps tnfa

Sujet: This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes HurTNFamediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17  kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25  kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome