ORAI1 anticorps (C-Term)

N° du produit ABIN3044087

Détail du produit anti-ORAI1 anticorps

Antigène: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Épitope: AA 278-301, C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ORAI1 est non-conjugé

Application: Western Blotting (WB)

Fonction: Rabbit IgG polyclonal antibody for Calcium release-activated calcium channel protein 1(ORAI1) detection. Tested with WB in Human,Mouse,Rat.

Séquence: EFARLQDQLD HRGDHPLTPG SHYA

Réactivité croisée (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Calcium release-activated calcium channel protein 1(ORAI1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: ORAI calcium release-activated calcium modulator 1
Protein Name: Calcium release-activated calcium channel protein 1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Orai1(278-301aa EFARLQDQLDHRGDHPLTPGSHYA), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for ORAI1 is approximately 2.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur ORAI1

Antigène: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Autre désignation: ORAI1 (ORAI1 Produits)

Synonymes: anticorps CRACM1, anticorps ORAT1, anticorps TMEM142A, anticorps RGD1311873, anticorps D730049H07Rik, anticorps Tmem142a, anticorps orai-1, anticorps im:7146264, anticorps orai1, anticorps tmem142a, anticorps zgc:109721, anticorps orai2, anticorps ORAI1, anticorps LOC100218031, anticorps ORAI calcium release-activated calcium modulator 1, anticorps ORAI calcium release-activated calcium modulator 1b, anticorps ORAI calcium release-activated calcium modulator 1 S homeolog, anticorps ORAI1, anticorps Orai1, anticorps orai1b, anticorps orai1.S, anticorps orai1

Sujet: ORAI1(ORAI calcium release-activated calcium modulator 1), also known as CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective ion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency(SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al.(2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp(E106D) and glu190-to-gln(E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion. Prakriya et al.(2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

Synonyms: Calcium release activated calcium channel protein 1 antibody|Calcium release activated calcium modulator 1 antibody|Calcium release-activated calcium channel protein 1 antibody|CRACM 1 antibody|CRACM1 antibody|CRCM1 antibody|CRCM1_HUMAN antibody|FLJ14466 antibody|Orai 1 antibody|ORAI calcium release activated calcium modulator 1 antibody|orai1 antibody|ORAT1 antibody|Protein orai 1 antibody|Protein orai-1 antibody|TMEM 142A antibody|TMEM142A antibody|Transmembrane protein 142A antibody

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling