XRCC4 anticorps (N-Term)

N° du produit ABIN3044561

Détail du produit anti-XRCC4 anticorps

Antigène: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Épitope: AA 49-75, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XRCC4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for DNA repair protein XRCC4(XRCC4) detection. Tested with WB, IHC-P in Human.

Séquence: ESEISQEADD MAMEKGKYVG ELRKALL

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for DNA repair protein XRCC4(XRCC4) detection. Tested with WB, IHC-P in Human.
Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 4
Protein Name: DNA repair protein XRCC4

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC4 (49-75aa ESEISQEADDMAMEKGKYVGELRKALL), different from the related mouse sequence by four amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur XRCC4

Antigène: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Autre désignation: XRCC4 (XRCC4 Produits)

Synonymes: anticorps zgc:73312, anticorps MGC81443, anticorps homolog of human DNA ligase iv-binding protein XRCC4, anticorps 2310057B22Rik, anticorps AW413319, anticorps AW545101, anticorps X-ray repair cross complementing 4, anticorps X-ray repair complementing defective repair in Chinese hamster cells 4, anticorps X-ray repair complementing defective repair in Chinese hamster cells 4 L homeolog, anticorps DNA ligase IV-binding protein, anticorps XRCC4, anticorps xrcc4, anticorps xrcc4.L, anticorps Xrcc4

Sujet: DNA repair protein XRCC4, also known as X-ray repair cross-complementing protein 4 or XRCC4, is a protein that in humans is encoded by the XRCC4 gene. In addition to humans, the XRCC4 protein is also expressed in many other metazoans, fungi and in plants. The X-ray repair cross-complementing protein 4 is one of several coreproteins involved in the non-homologous end joining (NHEJ) pathway to repair DNA double strand breaks(DSBs). Since XRCC4 is the key protein that enables interaction of LigIV to damaged DNA and therefore ligation of the ends, mutations in the XRCC4 gene were found to cause embryonic lethality in mice and developmental inhibition and immunodeficiency in humans. Furthermore, certain mutations in the XRCC4 gene are associated with an increased risk of cancer.

Synonyms: DNA double strand break repair and V(D)J recombination protein XRCC4 antibody|DNA repair protein XRCC4 antibody|X ray repair complementing defective repair in Chinese hamster cells 4 antibody|X ray repair cross complementing protein 4 antibody|X-ray repair cross-complementing protein 4 antibody|XRCC 4 antibody|XRCC4 antibody|XRCC4_HUMAN antibody

ID gène: 7518

UniProt: Q13426

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response