MMP 9 anticorps

N° du produit ABIN337099

Détail du produit anti-MMP 9 anticorps

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MMP 9 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: IHC-plus™

Specificité: Recognizes human MMP-9 at 92kD and 86kD (non-reducing conditions) which are identified as pro (latent) and active forms of matrix metalloproteinase-9 (MMP-9, also known as 92kD and collagenase IV or gelatinase B). Reacts with the complex (~200kD) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1)1. Does not cross-react with pro and active forms of MMP-2.

Purification: Protein G purified

Immunogène: Purified human gelatinase B. Cellular Localization: Peripheral cytoplasmic.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC, IHC-Fr (1:100), IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1 μg/mL for 2hrs at RT under non-reducing conditions. Immunoprecipitation: 2 μg/mg protein lysate. Use Protein G. Inhibition: 2-4 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MMP 9

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Autre désignation: MMP9 / Gelatinase B (MMP9 Produits)

Synonymes: anticorps CLG4B, anticorps GELB, anticorps MANDP2, anticorps MMP-9, anticorps AW743869, anticorps B/MMP9, anticorps Clg4b, anticorps pro-MMP-9, anticorps mmp-9, anticorps fgMMP-9, anticorps mmp9, anticorps ZFMMP-9, anticorps wu:fb02g06, anticorps wu:fb07b05, anticorps wu:fi98c09, anticorps wu:fj05a08, anticorps zgc:64165, anticorps clg4b, anticorps gelb, anticorps mandp2, anticorps matrix metallopeptidase 9, anticorps collagenase, anticorps matrix metalloproteinase 9, anticorps matrix metallopeptidase 9 S homeolog, anticorps MMP9, anticorps RB3913, anticorps BPSS0666, anticorps Sbal_3732, anticorps Bcer98_0486, anticorps Shew185_0630, anticorps Shal_3056, anticorps Sbal195_0657, anticorps Lbys_3550, anticorps Palpr_2084, anticorps Mmp9, anticorps mmp9, anticorps mmp9.S

Sujet: Name/Gene ID: MMP9
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP9, 92kd gelatinase, 92 kd type iv collagenase, 92 kDa gelatinase, 92 kDa type IV collagenase, Matrix metalloproteinase-9, MMP-9, Type V collagenase, CLG4B, Gelatinase B, GELB, Macrophage gelatinase, MANDP2

ID gène: 4318

UniProt: P14780

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events