PIK3CA anticorps (AA 878-891)

N° du produit ABIN337177

Cet anticorps Chèvre Polyclonal détecte spécifiquement PIK3CA dans IHC, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Lapin, Chien, Roussette (Chauve-souris), Hamster, Cheval et Singe.

Aperçu rapide pour PIK3CA anticorps (AA 878-891) (ABIN337177)

Antigène: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Chien, Roussette (Chauve-souris), Hamster, Cheval, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PIK3CA est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PIK3CA anticorps

Épitope: AA 878-891

Specificité: Human PIK3CA.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%) Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (93%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-HQWLKDKNKGEIYD from an internal region of human PIK3CA (NP_006209.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%), Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (93%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PIK3CA

Antigène: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Autre désignation: PIK3CA / PI3K

Sujet: Name/Gene ID: PIK3CA
Family: Non-Protein Kinase

Synonyms: PIK3CA, MCAP, p110-alpha, p110alpha, PI3K, PI3-kinase p110 subunit alpha, PI3-kinase subunit alpha, PI3Kalpha, MCM, MCMTC, PI3K-alpha, PtdIns-3-kinase subunit alpha, CLOVE

ID gène: 5290

NCBI Accession: NP_006209

UniProt: P42336

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling