Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificité
The antibody detects endogenous level of STAT1 only when phosphorylated at serine 727.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pSer727 (P-M-S (p) -P-E) derived from Human STAT1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Lan, Wu, Huang, Kuo, Wu, Liang, Chen: "High-glucose environment reduces human ?-defensin-2 expression in human keratinocytes: implications for poor diabetic wound healing." dans: The British journal of dermatology, Vol. 166, Issue 6, pp. 1221-9, (2012) (PubMed).
Antigène
STAT1
(Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
anticorps CANDF7, anticorps ISGF-3, anticorps STAT91, anticorps 2010005J02Rik, anticorps AA408197, anticorps XStat1, anticorps STAT4, anticorps DD6G4-4, anticorps STAT, anticorps STAT1, anticorps dZ182H3.3, anticorps si:by134g18.2, anticorps si:by51f19.1, anticorps si:dz182h3.3, anticorps si:dz199m19.1, anticorps si:dz73o4.2, anticorps stat1, anticorps signal transducer and activator of transcription 1, anticorps signal transducer and activator of transcription 1 L homeolog, anticorps signal transducer and activator of transcription 1, 91kDa, anticorps signal transducer and activator of transcription 4, anticorps signal transducer and activator of transcription 1a, anticorps STAT1, anticorps Stat1, anticorps stat1.L, anticorps stat4, anticorps stat1a
Sujet
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.