Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
Recognizes ORAI3, also known as Transmembrane Protein 142C, a 31.5 kDa multi-pass membrane protein belonging to the ORAI family. This antibody is not expected to cross react with either ORAI1 or ORAI2. Cellular Localization: Cell Membrane.
Réactivité croisée (Details)
Species reactivity (expected):Mouse and Rat. Species reactivity (tested):Human.
Purification
Affinity Chromatography.
Immunogène
ORAI3 antibody was raised against a 15 amino acid peptide from near the amino terminus of Human ORAI3
Western Blot: Detects a band of approximately 35 kDa in A20 cell lysates. Immunohistochemistry on Paraffin Sections: 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.02 % Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2.Synonyms: ORAI-3, TMEM142C, Transmembrane Protein 142C