HMGN1 anticorps (pSer20, pSer24)

N° du produit ABIN401321

Détail du produit anti-HMGN1 anticorps

Antigène: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Épitope: AA 19-28, pSer20, pSer24

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Poulet, Orang-Utan, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HMGN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions). Reactivity is seen against HMGN1 and HMGN2. Reactivity with non-phosphorylated human HMGNs is minimal.

Réactivité croisée (Details): Species reactivity (expected):Xenopus, Chicken, Mouse, Bovine, Canine, Orangutan, Rat, Porcine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding to amino acids 19-28 of human HMGN protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.20 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur HMGN1

Antigène: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Autre désignation: HMGN1 (HMGN1 Produits)

Synonymes: anticorps HMG14, anticorps hmg14, anticorps hmgn1, anticorps hmgn1b, anticorps HMG-14, anticorps Hmg14, anticorps high mobility group nucleosome binding domain 1, anticorps high mobility group nucleosome binding domain 1 S homeolog, anticorps high mobility group nucleosomal binding domain 1, anticorps non-histone chromosomal protein HMG-14, anticorps HMGN1, anticorps hmgn1.S, anticorps hmgn1, anticorps Hmgn1, anticorps LOC785479

Sujet: HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the HMG-box, that of the HMGN family, the nucleosomal binding domain, and that of the HMGA family, the AThook. The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as HMG motif proteins.Synonyms: HMG-14, HMG-17, HMG14, HMG17, HMGN1, HMGN2, Non-histone chromosomal protein HMG

ID gène: 3150

NCBI Accession: NP_004956

UniProt: P05114

Pathways: Chromatin Binding