TNF alpha anticorps (C-Term)

N° du produit ABIN4886747

Détail du produit anti-TNF alpha anticorps

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Épitope: AA 201-233, C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tumor necrosis factor
Protein Name: Tumor necrosis factor

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human TNF alpha(201-233aa QLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL), different from the related mouse sequence by five amino acids, and rat sequence by seven amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, The detection limit for TNF is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-TNF alpha (ABIN4886747)

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." dans: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." dans: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, (2015) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." dans: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Jiang, Han, Li, Yang, Liu: "Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo." dans: Carbohydrate polymers, Vol. 129, pp. 1-8, (2015) (PubMed).

Chen, Zhao, Wu, Zou, Luo, Li, Xie, Liang: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." dans: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).

Li, Zhang, Fu, Sun, Gong, Jiang, Zhang: "Dual pancreas- and lung-targeting therapy for local and systemic complications of acute pancreatitis mediated by a phenolic propanediamine moiety." dans: Journal of controlled release : official journal of the Controlled Release Society, Vol. 212, pp. 19-29, (2015) (PubMed).

Li, Chen, Zhang, Song, Mu: "Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats." dans: Neural regeneration research, Vol. 7, Issue 5, pp. 325-31, (2015) (PubMed).

Li, Qiu, Wang, Niu, Pan: "Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury." dans: Food & function, Vol. 6, Issue 2, pp. 470-8, (2015) (PubMed).

Xia, Li, Xiang, Chang, Xia, Han: "Neuroprotective effects of an aqueous extract of Futokadsura stem in an A?-induced Alzheimer's disease-like rat model." dans: The Chinese journal of physiology, Vol. 58, Issue 2, pp. 104-13, (2015) (PubMed).

Xie, Zhang, Li, Xu: "Inhibition of inflammatory cytokines after early decompression may mediate recovery of neurological function in rats with spinal cord injury." dans: Neural regeneration research, Vol. 10, Issue 2, pp. 219-24, (2015) (PubMed).

Wu, Su, Li, Wu, Wu, Tang: "Cantharidin-induced liver injuries in mice and the protective effect of vitamin C supplementation." dans: International immunopharmacology, Vol. 28, Issue 1, pp. 182-7, (2015) (PubMed).

Wu, Hu, Liu, Cao, Xu, Li, Li: "Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice." dans: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1498-506, (2014) (PubMed).

Li, Qian, Ju, Wang: "Upregulation of Toll-like receptor 2 expression in colorectal cancer infected by human cytomegalovirus." dans: Oncology letters, Vol. 9, Issue 1, pp. 365-370, (2014) (PubMed).

Liu, Zeng, Liu, Wang, Maitz, Wang, Liu, Chen, Huang: "Surface modification with dopamine and heparin/poly-L-lysine nanoparticles provides a favorable release behavior for the healing of vascular stent lesions." dans: ACS applied materials & interfaces, Vol. 6, Issue 11, pp. 8729-43, (2014) (PubMed).

Huang, Du, Fan, Lv, Qian, Zhang, Han, Chen, Wu, Jin: "The effect of proinflammatory cytokines on IL-17RA expression in NSCLC." dans: Medical oncology (Northwood, London, England), Vol. 31, Issue 9, pp. 144, (2014) (PubMed).

Ye, Zheng, Fan, Qin, Li, Tang, Ai, Zhang, Bian, Dang, Huang, Zhou, Zhou, Xiong, Yan, Ma, Li: "Lactoferrin deficiency promotes colitis-associated colorectal dysplasia in mice." dans: PLoS ONE, Vol. 9, Issue 7, pp. e103298, (2014) (PubMed).

Wang, Zhou, Chen, Zhu: "Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1? expression." dans: Brazilian journal of medical and biological research = Revista brasileira de pesquisas me?dicas e biolo?gicas / Sociedade Brasileira de Biofi?sica ... [et al.], Vol. 47, Issue 9, pp. 738-45, (2014) (PubMed).

Zhao, Li, Li, Liu, Liu, Li, Xiao, Li: "Long-term resveratrol treatment prevents ovariectomy-induced osteopenia in rats without hyperplastic effects on the uterus." dans: The British journal of nutrition, Vol. 111, Issue 5, pp. 836-46, (2014) (PubMed).

Hou, Zhu, Pang, Jeffry, Zhou: "Protective effect of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats induced by yeast and potassium oxonate." dans: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 64, pp. 57-64, (2014) (PubMed).

Jiang, Chen, Li, An, Jiao: "Ulinastatin attenuates renal interstitial inflammation and inhibits fibrosis progression in rats under unilateral ureteral obstruction." dans: Molecular medicine reports, Vol. 10, Issue 3, pp. 1501-8, (2014) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF (TNF alpha Produits)

Synonymes: anticorps DIF, anticorps TNF-alpha, anticorps TNFA, anticorps TNFSF2, anticorps RATTNF, anticorps Tnfa, anticorps tnf, anticorps TNF-a, anticorps TNFalpha, anticorps Tnfsf1a, anticorps TNFa, anticorps cTNF, anticorps Tnf-alpha, anticorps tnfa-like, anticorps TNF-ALPHA, anticorps dif, anticorps tnfa, anticorps xtnf, anticorps tnfsf2, anticorps tnf-alpha, anticorps Cachectin, anticorps tumor necrosis factor, anticorps tumor necrosis factor b (TNF superfamily, member 2), anticorps tumor necrosis factor alpha, anticorps tumor necrosis factor a (TNF superfamily, member 2), anticorps TNF, anticorps Tnf, anticorps tnf, anticorps tnfb, anticorps tnf-alpha, anticorps LOC103694380, anticorps tnfa

Sujet: TNF alpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Synonyms: APC1 antibody|APC1 protein antibody|Cachectin antibody|DIF antibody|Differentiation inducing factor antibody|Macrophage cytotoxic factor antibody|MCF antibody|Necrosin antibody|TNF a antibody|TNF alpha antibody|TNF antibody|TNF Macrophage Derived antibody|TNF Monocyte Derived antibody|TNF Superfamily Member 2 antibody|TNF superfamily, member 2 antibody|TNF, macrophage derived antibody|TNF, monocyte derived antibody|TNF-a antibody|TNF-alpha antibody|TNFA antibody|TNFSF2 antibody|Tumor necrosis factor alpha antibody|Tumor necrosis factor antibody|Tumor necrosis factor ligand superfamily member 2 antibody|Tumor Necrosis Factor Precursor antibody|Tumor Necrosis Factor, Membrane Form antibody|Tumor necrosis factor, soluble form antibody|Tumour Necrosis Factor Alpha antibody

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome