WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The catenins, alpha, beta and gamma, are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3beta, result in accumulation of the beta-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of beta-catenin signaling that directly binds a phosphorylated Ser-ro motif next to the APC-binding site in beta-catenin, inhibiting the interaction with APC and increasing beta-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of beta-catenin in tumors such as breast cancer.Synonyms: Beta-catenin, CTNNB, CTNNB1