FEN1 anticorps (AA 243-272)

N° du produit ABIN5534280

Détail du produit anti-FEN1 anticorps

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Épitope: AA 243-272

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FEN1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogène: This FEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human FEN1.

Isotype: Ig Fraction

Information d'application

Indications d'application: For IF starting dilution is: 1:100

For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur FEN1

Antigène: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Autre désignation: FEN1 (FEN1 Produits)

Synonymes: anticorps FEN-1, anticorps FEN1, anticorps AW538437, anticorps MF1, anticorps RAD2, anticorps cb879, anticorps fen-1, anticorps fen1, anticorps mf1, anticorps rad2, anticorps xFEN1a, anticorps flap structure-specific endonuclease 1, anticorps flap endonuclease 1-like, anticorps Flap endonuclease-1, anticorps 5'-3' exonuclease family protein, anticorps flap structure specific endonuclease 1, anticorps flap structure-specific endonuclease 1 L homeolog, anticorps nuclease, Rad2 family, anticorps FEN1, anticorps LOC100808044, anticorps Bm1_49605, anticorps AT5G26680, anticorps Fen1, anticorps fen1, anticorps fen1.L

Sujet: FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. This protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Poids moléculaire: 43 kDa

ID gène: 2237

UniProt: P39748

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA