TNFSF11
Reactivité: Rat
WB, IHC, IP, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA:
To detect hsRANKL by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hsRANKL.
Sandwich:
To detect hsRANKL by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sRANKL (XP-5273) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.
Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Stock
-20 °C
Stockage commentaire
sRANK Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Antigène
RANKL (TNFSF11)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))