TNFRSF1A anticorps

N° du produit ABIN5706820

Détail du produit anti-TNFRSF1A anticorps

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivité: Humain, Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNFRSF1A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Attributs du produit:

Synonyms: TBP1 antibody, TNFR1 antibody, TNFR55 antibody, TNFRSF1A antibody, Tumor necrosis factor alpha receptor antibody, rabbit anti-Tumor Necrosis Factor p55 Antibody, rabbit anti-TNF p55 Antibody

Background: Tumor necrosis factor receptor superfamily member 1A (TNF p55 Receptor) is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. It contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

Gene Name: TNFR-I

Purification: This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against the extracellular domain of the cell bound human TNFRp55.  In general, this antibody also detects primate sTNFRp55.  The antibody does not recognize human sTNFRp75.  In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the TNFRp55 found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.

Immunogène:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human sTNFRp55 expressed in Chinese Hamster Cells.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application:

Application Note: This antiserum against Human TNF p55 Receptor (sTNFRp55) has been tested for use in ELISA,  radioimmunoassays, immunoblotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.This product has been assayed for the ability to immunoprecipitate antigen.  A dilution range of 1:400 to 1:800 is suggested for this immunoassay.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  This product has been assayed by immunoblot  using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application.  This product has been assayed by ELISA against soluble (extracellular) sTNFRp55 using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2,000 is suggested for this product.  For use in ELISA formats, this antibody is best used as the second antibody  in combination with a monoclonal antibody as a capture antibody.  This product has been assayed by radioimmunoassay against antigen.  A dilution of 1:8,000 is suggested for this immunoassay.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:400 to 1:800

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 85 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Détails sur TNFRSF1A

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Autre désignation: TNF p55 Receptor (TNFRSF1A Produits)

Synonymes: anticorps CD120a, anticorps FPF, anticorps MS5, anticorps TBP1, anticorps TNF-R, anticorps TNF-R-I, anticorps TNF-R55, anticorps TNFAR, anticorps TNFR1, anticorps TNFR1-d2, anticorps TNFR55, anticorps TNFR60, anticorps p55, anticorps p55-R, anticorps p60, anticorps TNF-R1, anticorps TNF-alphaR1, anticorps TNFRI, anticorps TNFRp55, anticorps TNFalpha-R1, anticorps Tnfr-2, anticorps Tnfr1, anticorps fpf, anticorps tbp1, anticorps p55-r, anticorps tnf-r, anticorps tnfar, anticorps tnfr1, anticorps cd120a, anticorps tnfr55, anticorps tnfr60, anticorps xtnfr1, anticorps wu:fj65b10, anticorps zgc:85914, anticorps TNFR I, anticorps TNFRSF1A, anticorps TNFA, anticorps cTNF, anticorps TNF receptor superfamily member 1A, anticorps tumor necrosis factor receptor superfamily, member 1a, anticorps TNF receptor superfamily member 1A S homeolog, anticorps tumor necrosis factor, anticorps TNFRSF1A, anticorps Tnfrsf1a, anticorps tnfrsf1a.S, anticorps tnfrsf1a, anticorps TNF

ID gène: 7132

UniProt: P19438

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway