CD11b anticorps

N° du produit ABIN573960

Détail du produit anti-CD11b anticorps

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain, Souris, Lapin

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

Purification: Ion exchange chromatography

Immunogène: T cell enriched splenocytes from B10 mice.

Type of Immunogen: Cells

Clone: M1-70-15

Isotype: IgG2b

Information d'application

Indications d'application: Approved: Flo (1:50), IHC, IHC-Fr, IHC-P (10 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: ITGAM / CD11b (ITGAM Produits)

Synonymes: anticorps CD11b/CD18, anticorps CR3, anticorps CR3A, anticorps Cd11b, anticorps F730045J24Rik, anticorps Ly-40, anticorps MAC1, anticorps Mac-1, anticorps Mac-1a, anticorps CD11B, anticorps MAC-1, anticorps MAC1A, anticorps MO1A, anticorps SLEB6, anticorps C3bi, anticorps integrin subunit alpha M, anticorps integrin alpha M, anticorps integrin, alpha M (complement component 3 receptor 3 subunit), anticorps integrin alpha-M, anticorps ITGAM, anticorps Itgam, anticorps LOC100351865

Sujet: Name/Gene ID: ITGAM
Family: Integrin

Synonyms: ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain

ID gène: 3684

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation