PRAS40 anticorps (Internal Region)
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- Antigène Voir toutes PRAS40 (AKT1S1) Anticorps
- PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
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Épitope
- Internal Region
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PRAS40 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificité
- PRAS40 Antibody detects endogenous levels of total PRAS40.
- Homologie
- Pig,Bovine,Horse,Sheep,Dog
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogène
- A synthesized peptide derived from human PRAS40, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product AKT1S1 Anticorps primaire
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- Indications d'application
- WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20 °C. Stable for 12 months from date of receipt.
- Date de péremption
- 12 months
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- Antigène
- PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
- Autre désignation
- AKT1S1 (AKT1S1 Produits)
- Synonymes
- anticorps MGC81452, anticorps lobe, anticorps pras40, anticorps fb34a04, anticorps wu:fb34a04, anticorps Lobe, anticorps PRAS40, anticorps 1110012J22Rik, anticorps AI227026, anticorps Lobel, anticorps AKT1 substrate 1 (proline rich) S homeolog, anticorps AKT1 substrate 1, anticorps AKT1 substrate 1 (proline rich), anticorps AKT1 substrate 1 (proline-rich), anticorps akt1s1.S, anticorps AKT1S1, anticorps akt1s1, anticorps Akt1s1
- Sujet
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Description: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Gene: AKT1S1
- Poids moléculaire
- 27 kDa
- ID gène
- 84335
- UniProt
- Q96B36
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, L'effet Warburg
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