BRCA1 anticorps (AA 1360-1440)

N° du produit ABIN6281204

Détail du produit anti-BRCA1 anticorps

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Épitope: AA 1360-1440

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: BRCA1 is a protein encoded by the BRCA1 gene which is approximately 207,7 kDa. BRCA1 is localised to the cytoplasm and nucleus. It is involved in DNA double-strand break repair and meiosis. It is a E3 ubiquitin-protein ligasespecifically mediates the formation of Lys-6-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. The BRCA1-BARD1 heterodimer coordinates a range of cellular pathways such as ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 isoform 1 and 3 are widely expressed. Mutations in the BRCA1 gene may result in pancreatic cancer and breast cancer. STJ91884 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of BRCA1 protein.

Specificité: BRCA1 Polyclonal Antibody detects endogenous levels of BRCA1 protein.

Attributs du produit: Rabbit polyclonal to BRCA1.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human BRCA1 around the non-phosphorylation site of S1423.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000

Commentaires:

Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C, and avoid repeat freeze-thaw cycles.

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1 (BRCA1 Produits)

Synonymes: anticorps BRCAI, anticorps BRCC1, anticorps BROVCA1, anticorps IRIS, anticorps PNCA4, anticorps PPP1R53, anticorps PSCP, anticorps RNF53, anticorps iris, anticorps pscp, anticorps brcai, anticorps brcc1, anticorps rnf53, anticorps brca1, anticorps BRCA1, anticorps LOC100224649, anticorps BRCA1, DNA repair associated, anticorps breast cancer 1, anticorps breast cancer 1, early onset, anticorps breast cancer 1 L homeolog, anticorps BRCA1, anticorps brca1, anticorps LOC755086, anticorps Brca1, anticorps brca1.L

Poids moléculaire: 180 kDa

ID gène: 672

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus