CDT1 anticorps

N° du produit ABIN6942498

Détail du produit anti-CDT1 anticorps

Antigène: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CDT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée: Humain

Réactivité croisée (Details): WB(1:500-1000), IHC-P(1:25-50)

Purification: Purified by Protein A.

Immunogène: Human CDT1 aa 100 to the C-terminus

Clone: 2H5

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur CDT1

Antigène: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Autre désignation: CDT1 (CDT1 Produits)

Synonymes: anticorps CDT1, anticorps fc37h07, anticorps im:7158083, anticorps wu:fc37h07, anticorps wu:fi38h09, anticorps xcdt1, anticorps DUP, anticorps RIS2, anticorps 2610318F11Rik, anticorps AW545653, anticorps C76791, anticorps Ris2, anticorps dup, anticorps ris2, anticorps chromatin licensing and DNA replication factor 1, anticorps chromatin licensing and DNA replication factor 1 L homeolog, anticorps CDT1, anticorps cdt1, anticorps Cdt1, anticorps cdt1.L

Sujet:

Synonyms: DNA replication factor Cdt1, Double parked homolog, DUP, CDT1, chromatin licensing and DNA replication factor 1.

Background: Human Cdt1 is a nuclear localizing replication initiation factor that is expressed only during the G1 and S phases of the cell cycle. In conjunction with Cdc18, Cdt1 is required to load the MCM protein Cdc21 onto chromatin at the end of mitosis which is necessary to initiate DNA replication. After S-phase onset, Cdt1 protein levels decrease and are barely detectable in cells in early S-phase or G2. However, Cdt1 mRNA is expressed in S-phase-arrested cells, and its levels do not change dramatically during the cell cycle, suggesting that proteolytic degradation rather than transcriptional controls ensure proper accumulation of Cdt1. Cdt1 can associate with the DNA replication inhibitor geminin, which is present in the S and G2 phases of the cell cycle. Inhibition of DNA replication by geminin in cell-free DNA replication extracts can be reversed by the addition of excess Cdt1. Geminin may be responsible for preventing inappropriate origin firing by targeting Cdt1.

ID gène: 81620

UniProt: Q9H211

Pathways: Signalisation MAPK, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA