CD45 anticorps (violetFluor™ 450)
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- Antigène Voir toutes CD45 (PTPRC) Anticorps
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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Reactivité
- Humain, Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD45 est conjugé à/à la violetFluor™ 450
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Application
- Flow Cytometry (FACS)
- Réactivité croisée
- Cat
- Purification
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- Clone
- RA3-6B2
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product PTPRC Anticorps primaire
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- Indications d'application
- This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.
- Commentaires
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0.2 mg/mL
- Restrictions
- For Research Use only
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- 2-8°C protected from light
- Date de péremption
- 12 months
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- Antigène
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- Autre désignation
- CD45R (B220) (PTPRC Produits)
- Synonymes
- anticorps PTPRC, anticorps si:dkeyp-47c5.1, anticorps B220, anticorps CD45, anticorps CD45R, anticorps GP180, anticorps L-CA, anticorps LCA, anticorps LY5, anticorps T200, anticorps Cd45, anticorps Ly-5, anticorps Lyt-4, anticorps loc, anticorps Lca, anticorps RT7, anticorps cd45, anticorps protein tyrosine phosphatase, receptor type C, anticorps protein tyrosine phosphatase, receptor type, C, anticorps receptor-type tyrosine-protein phosphatase C, anticorps PTPRC, anticorps ptprc, anticorps LOC100074271, anticorps Ptprc
- Sujet
- The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/ B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.
- ID gène
- 19264, 5788
- UniProt
- P08575, P06800
- Pathways
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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