Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
sRANK Ligand Antibody is affinity chromatography purified via peptide column.
Immunogène
Anti-sRANK-L antibody was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L.
TNFSF11
Reactivité: Rat
WB, IHC, IP, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 0.25-4 μ,g/mL, IHC: 5 μ,g/mL.
Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
sRANK Ligand Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
sRANK Ligand antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
RANKL (TNFSF11)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
SRANK Ligand Antibody: The receptor activator of NF-κ,B ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.