Glycogen Synthase Kinase 3 (GSK3) (pTyr216), (pTyr279) anticorps

N° du produit ABIN7138967

Cet anticorps Lapin Polyclonal détecte spécifiquement dans ELISA, WB, IHC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour Glycogen Synthase Kinase 3 (GSK3) (pTyr216), (pTyr279) anticorps (ABIN7138967)

Antigène: Glycogen Synthase Kinase 3 (GSK3)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit

Épitope: pTyr216, pTyr279

Réactivité croisée: Humain, Souris, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogène: Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3alpha/beta .

Isotype: IgG

Information d'application

Indications d'application: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détail du antigène

Antigène: Glycogen Synthase Kinase 3 (GSK3)

Autre désignation: GSK3A/GSK3B

Sujet:

Background: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13, 137(1-2): 193-201 Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02, 316(2): 411-415 Fan G, et al. J Biol Chem 2003 Dec 26, 278(52): 52432-52436 Liao X, et al. Mol Cancer Ther 2003 Nov, 2(11): 1215-1222

Aliases: Factor A, GSK-3 alpha/beta, kinase GSK3-alpha/beta

UniProt: P49840, P49841