SNCA anticorps (AA 117-131)
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- Antigène Voir toutes SNCA Anticorps
- SNCA (Synuclein, alpha (SNCA))
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Épitope
- AA 117-131
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Reactivité
- Humain, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp SNCA est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificité
- This antibody has been shown to react strongly with human brain-derived α-synuclein. The antibody also reacts with rat brain-derived α-synuclein but reactivity appears substantially less than with human.
- Réactivité croisée (Details)
- Does not cross-react with mouse α-synuclein.
- Attributs du produit
- NACP| Non-A β component of senile plaques,alpha-Synuclein polyclonal antibody
- Purification
- Purified by caprylic acid and ammonium sulphate precipitation.,Purified from rabbit serum. Vial contains an immunoglobulin preparation, partially purified by chromatography from whole serum, suspended in phosphate-buffered saline (pH 7.4) containing 0.01M sodium azide.
- Immunogène
- Synthetic peptide corresponding to [Cys] + aa 117-131 of human alpha-synuclein conjugated to KLH. The sequence is conserved in rat alpha-synuclein except for two substitutions, namely Asp121Ser and Asn122Ser.
- Top Product
- Discover our top product SNCA Anticorps primaire
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- Indications d'application
- Western blot (1:1,000-1:20,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.,Western blot: The antibody has been characterized by single dimension SDS-PAGE using recombinant α- and β-synucleins as well as rat, mouse and human brain lysates with and without peptide blockade. Studies with the recombinant proteins demonstrated the absolute specificity of the antibody for α-synuclein. The antibody gives a strong band at a relative molecular weight of approximately 15 kDa with human brain lysate which is wholly abolished by preincubation with the immunizing peptide (Prod. No. BML-SP9340).Immunohistochemistry: The antibody has been tested on de-waxed tissue sections of human brain fixed in neutral buffered formaldehyde. A standard protocol should be followed.
- Commentaires
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For Blocking peptide see Prod. No. BML-SP9340.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Liquid. In PBS, pH 7.4, containing 10 mM sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 °C.
- Stock
- -20 °C
- Stockage commentaire
- Store unopened vial at -20°C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality 'antiserum-grade' plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
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- Antigène
- SNCA (Synuclein, alpha (SNCA))
- Autre désignation
- alpha-Synuclein (SNCA Produits)
- Synonymes
- anticorps snca, anticorps MGC64356, anticorps LOC619283, anticorps NACP, anticorps alphaSYN, anticorps PARK1, anticorps PARK4, anticorps PD1, anticorps synuclein alpha L homeolog, anticorps alpha-synuclein, anticorps synuclein alpha, anticorps synuclein, alpha, anticorps snca.L, anticorps LOC619283, anticorps SNCA, anticorps Snca
- UniProt
- P37840
- Pathways
- Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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