IL-1 beta anticorps (N-Term)

N° du produit ABIN799612

Détail du produit anti-IL-1 beta anticorps

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Épitope: N-Term

Reactivité: Humain, Chien, Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-1 beta est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).

Purification: Purified IgG

Immunogène: Recombinant human IL1B / IL1 Beta.

Type of Immunogen: Recombinant protein

Information d'application

Indications d'application: Approved: ELISA (1:500 - 1:2000), IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), RIA (1:5000 - 1:10000), WB (1:100 - 1:200)

Usage: This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Détails sur IL-1 beta

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: IL1B (IL1B Produits)

Synonymes: anticorps IL-1, anticorps IL1-BETA, anticorps IL1F2, anticorps IL-1BETA, anticorps IL1beta, anticorps il1-b, anticorps zgc:111873, anticorps IL-1B, anticorps IL-1beta, anticorps Il-1b, anticorps IL1B, anticorps IL-1 beta, anticorps IL-1b, anticorps interleukin 1 beta, anticorps interleukin 1, beta, anticorps IL1B, anticorps il1b, anticorps Il1b

Sujet: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

ID gène: 3553

UniProt: P01584

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome