SMAD2 anticorps (pSer465, pSer467) (Alexa Fluor 488)
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- Antigène Voir toutes SMAD2 Anticorps
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
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Épitope
- pSer465, pSer467
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp SMAD2 est conjugé à/à la Alexa Fluor 488
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Réactivité croisée
- Humain, Souris, Rat
- Homologie
- Dog,Cow,Horse,Chicken
- Purification
- Purified by Protein A.
- Immunogène
- KLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467
- Isotype
- IgG
- Top Product
- Discover our top product SMAD2 Anticorps primaire
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- Indications d'application
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- Autre désignation
- Smad2 (SMAD2 Produits)
- Synonymes
- anticorps JV18, anticorps JV18-1, anticorps MADH2, anticorps MADR2, anticorps hMAD-2, anticorps hSMAD2, anticorps jv18, anticorps Xmad2, anticorps madh2, anticorps madr2, anticorps XSmad2, anticorps hmad-2, anticorps hsmad2, anticorps jv18-1, anticorps smad-2, anticorps fj43c06, anticorps wu:fj43c06, anticorps 7120426M23Rik, anticorps Madh2, anticorps Madr2, anticorps Smad-2, anticorps mMad2, anticorps MAD homolog 2, anticorps Mothers against DPP homolog 2, anticorps SMAD 2, anticorps Smad2, anticorps SMAD family member 2, anticorps SMAD family member 2-Z, anticorps SMAD family member 2 S homeolog, anticorps SMAD2, anticorps SMAD2Z, anticorps smad2, anticorps smad2.S, anticorps Smad2
- Sujet
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Synonyms: Smad2p-Ser465/467, p-Smad2Ser465/467, Smad2p-S465/467, hMAD 2, hSMAD2, JV18 1, JV18, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, mothers against DPP homolog 2, SMAD 2, SMAD, SMAD2, SMAD2_HUMAN.
Background: Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8), the common partner, (Smad4) that functions via its interaction to the various Smad's, and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.
- ID gène
- 4087
- Pathways
- Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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