Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)
Specificité
The antibody MEM-150 reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored extracellular glycoprotein expressed on various cell types including erythrocytes, lymphoblasts, at low levels it is present on circulating lymphocytes.
Réactivité croisée (Details)
Human
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
SEMA7A
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow cytometry: Recommended dilution: 4 μg/mL. Western blotting: Non-reducing conditions.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Angelisová, Drbal, Cerný, Hilgert, Horejsí: "Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules." dans: Immunobiology, Vol. 200, Issue 2, pp. 234-45, (1999) (PubMed).
Mudad, Rao, Angelisova, Horejsi, Telen: "Evidence that CDw108 membrane protein bears the JMH blood group antigen." dans: Transfusion, Vol. 35, Issue 7, pp. 566-70, (1995) (PubMed).
Semaphorin 7A (John Milton Hagen blood group),CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors –, plexin C1 and beta1 integrins.,Semaphorin 7a, JMH blood group antigen, SEMAL, SEMAK1