PIK3CA anticorps (AA 101-300)

N° du produit ABIN968519

L’anticorps Souris Monoclonal anti-PIK3CA a été validé pour WB et IF. Il convient pour détecter PIK3CA dans des échantillons de Humain et Rat. Il y a 5+ publications disponibles.

Aperçu rapide pour PIK3CA anticorps (AA 101-300) (ABIN968519)

Antigène: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PIK3CA est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 19-PI3

Détail du produit anti-PIK3CA anticorps

Épitope: AA 101-300

Réactivité croisée: Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human PI3-Kinase p110alpha aa. 101-300

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-PIK3CA (ABIN968519)

Funaki, Katagiri, Kanda, Anai, Nawano, Ogihara, Inukai, Fukushima, Ono, Yazaki, Kikuchi, Oka, Asano: "p85/p110-type phosphatidylinositol kinase phosphorylates not only the D-3, but also the D-4 position of the inositol ring." dans: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 22019-24, (1999) (PubMed).

Vicente-Manzanares, Rey, Jones, Sancho, Mellado, Rodriguez-Frade, del Pozo, Yáñez-Mó, de Ana, Martínez-A, Mérida, Sánchez-Madrid: "Involvement of phosphatidylinositol 3-kinase in stromal cell-derived factor-1 alpha-induced lymphocyte polarization and chemotaxis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 7, pp. 4001-12, (1999) (PubMed).

Katagiri, Asano, Ishihara, Inukai, Shibasaki, Kikuchi, Yazaki, Oka: "Overexpression of catalytic subunit p110alpha of phosphatidylinositol 3-kinase increases glucose transport activity with translocation of glucose transporters in 3T3-L1 adipocytes." dans: The Journal of biological chemistry, Vol. 271, Issue 29, pp. 16987-90, (1996) (PubMed).

Hu, Klippel, Muslin, Fantl, Williams: "Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase." dans: Science (New York, N.Y.), Vol. 268, Issue 5207, pp. 100-2, (1995) (PubMed).

Volinia, Hiles, Ormondroyd, Nizetic, Antonacci, Rocchi, Waterfield: "Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene." dans: Genomics, Vol. 24, Issue 3, pp. 472-7, (1995) (PubMed).

Détails sur PIK3CA

Antigène: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Autre désignation: PI3-Kinase p110 alpha

Sujet: Phosphatidylinositol 3 (PI3) -kinase participates in insulin-stimulated glucose uptake, PDGF-induced membrane ruffling, and G-protein receptor signaling. It exists as a heterodimer of 85 kDa (p85) and 110 kDa (p110) subunits. The p85 subunit associates with and serves as a substrate for activated growth factor receptor tyrosine kinases. p85 regulates the p110 catalytic subunit by acting as the link between PI3-kinase and the ligand-activated receptor. Four isoforms of p110 have been identified (alpha, beta, gamma, and delta). The p110alpha isoform contains an N-terminal region involved in p85 binding and a C-terminal kinase domain. p85/p110alpha-type PI kinase phosphorylates the D-3 and D-4 position of the inositol ring of PI, thereby producing PtdIns(3)P, PtdIns(3,4)P[2], PtdIns(3,4,5)P[3], PtdIns(4)P, and PtdIns(4,5) P[2]. During induction of chemotaxis by the chemokine SDF-1alpha, PI3-kinase regulates adhesion and ERM protein redistribution in the lymphocyte plasma membrane. In addition, PI3-kinases activate other signaling molecules, such as p70 S6 kinase and Akt/protein kinase B. Thus, p85/p110alpha-type PI kinase is a ubiquitously expressed kinase that is involved in a variety of cell signaling cascades.

Poids moléculaire: 110 kDa

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling