anti-Poulet alpha Tubulin anticorps pour Fluorescence Microscopy

Recommended alpha Tubulin Antibody

Antigène
alpha Tubulin (TUBA1) Anticorps
  • Tuba1
  • K-ALPHA-1
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 1b
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • tuba1b
  • Tuba1b
Épitope
C-Term
31
28
17
12
11
9
9
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus), Poulet, Boeuf (Vache)
297
188
177
41
35
34
26
19
18
17
13
10
10
10
8
8
8
8
8
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Hôte
Souris
184
133
24
5
2
2
2
1
Clonalité
Monoclonal
Conjugué
Cet anticorp alpha Tubulin est non-conjugé
22
10
8
8
8
8
8
8
8
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
266
110
102
85
73
66
55
31
18
16
13
8
3
2
1
1
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N° du produit ABIN964563
$ 493.50
Plus frais de port $45.00 et $22.60 pour carboglace

General

Antigène alpha Tubulin (TUBA1) Anticorps
Épitope C-Term
(31), (28), (17), (12), (11), (9), (9), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité Humain, Souris, Rat (Rattus), Poulet, Boeuf (Vache)
(297), (188), (177), (41), (35), (34), (26), (19), (18), (17), (13), (10), (10), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Hôte Souris
(184), (133), (24), (5), (2), (2), (2), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp alpha Tubulin est non-conjugé
(22), (10), (8), (8), (8), (8), (8), (8), (8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(266), (110), (102), (85), (73), (66), (55), (31), (18), (16), (13), (8), (3), (2), (1), (1)
Pubmed 5 références disponible
Fournisseur

Détail du produit anti-alpha Tubulin anticorps

Détail du antigène alpha Tubulin Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN964563) Images
Specificité Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin.  A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
 Réactivité croisée Souris, Rat (Rattus), Poulet, Mouton
Attributs du produit Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Stérilité Sterile filtered
Immunogène Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of of human alpha tubulin protein.
Clone 17H11-F10
Isotype IgG1 kappa
Plasmids, Primers & others

Détail du antigène alpha Tubulin

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Antigène
Autre désignation Alpha-Tubulin (TUBA1 Antibody Extrait)
Sujet Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.
Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous Tubulin K-alpha-1, TUBA1B, tubulin loading control
ID gène 17986283
UniProt P68363
Pathways Dynamique des Microtubules

Information d'application

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Stockage References for anti-alpha Tubulin antibody (ABIN964563) Images Haut de la page
Indications d'application Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Commentaires

Gene Name: TUBA1B

Restrictions For Research Use only

Stockage

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application References for anti-alpha Tubulin antibody (ABIN964563) Images Haut de la page
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Date de péremption 12 months

References for anti-alpha Tubulin antibody (ABIN964563)

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application Stockage Images Haut de la page
Produit citée dans:

Chavez, Coricor, Perez, Seo, Serra: "SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes." dans: Osteoarthritis and cartilage, Vol. 25, Issue 2, pp. 332-340, 2018

Patel, Blaauboer, Tucker, Mansouri, Ruiz-Moreno, Hamann, Schumann, Opitz, Jin: "The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 2, pp. 776-787, 2017

Roy, Jerman, Jozsef, McNamara, Onyekaba, Sun, Marin: "Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation." dans: The Journal of biological chemistry, Vol. 292, Issue 43, pp. 17703-17717, 2017

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." dans: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, 2015

Blaauboer, Gabrielle, Jin: "MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 492-502, 2014

Images

Détail du produit anti-alpha Tubulin anticorps Détail du antigène alpha Tubulin Information d'application Stockage References for anti-alpha Tubulin antibody (ABIN964563) Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) (C-Term) antibody (ABIN964563) HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loa...
 image for anti-alpha Tubulin (TUBA1) (C-Term) antibody (ABIN964563) anti-alpha Tubulin (TUBA1) (C-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) (C-Term) antibody (ABIN964563) Immunofluorescence of Mouse monoclonal anti-Alpha Tubulin antibody. Cell Type: A431 c...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) (C-Term) antibody (ABIN964563) Immunofluorescences of Mouse monoclonal anti-Alpha Tubulin antibody. Cell Type: A431 ...