TRAIL Kit ELISA
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- Antigène Voir toutes TRAIL (TNFSF10) Kits ELISA
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6-1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Fonction
- For quantitative detection of TRAIL in Human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Sensibilité
- < 1 pg/mL
- Ingrédients
- 1. One 96-well plate pre-coated with anti-Human TRAIL antibody 2. Lyophilized Human TRAIL standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human TRAIL antibody (Concentrated): 130 µl.
- Matériel non inclus
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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- Commentaires
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TRAIL polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TRAIL polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the TRAIL amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of TRAIL can be calculated.
- Plaque
- Pre-coated
- Préparation des réactifs
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- Préparation de l'échantillon
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants, tissue lysate or body fluids: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C° C.
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with citrate, heparin or EDTA as the anticoagulant. Centrifuge for 15min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - Restrictions
- For Research Use only
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- Agent conservateur
- Sodium azide, Thimerosal (Merthiolate)
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- Antigène Voir toutes TRAIL (TNFSF10) Kits ELISA
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Autre désignation
- TRAIL (TNFSF10 Produits)
- Synonymes
- APO2L Kit ELISA, Apo-2L Kit ELISA, CD253 Kit ELISA, TL2 Kit ELISA, TRAIL Kit ELISA, A330042I21Rik Kit ELISA, AI448571 Kit ELISA, APO-2L Kit ELISA, Ly81 Kit ELISA, Trail Kit ELISA, tl2 Kit ELISA, apo2l Kit ELISA, cd253 Kit ELISA, trail Kit ELISA, apo-2l Kit ELISA, trail1 Kit ELISA, xtrail1 Kit ELISA, tnfsf10l2 Kit ELISA, zgc:92320 Kit ELISA, TNLG6A Kit ELISA, TNF superfamily member 10 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 10 Kit ELISA, tumor necrosis factor superfamily member 10 Kit ELISA, TNF superfamily member 10 S homeolog Kit ELISA, TNFSF10 Kit ELISA, Tnfsf10 Kit ELISA, tnfsf10.S Kit ELISA, tnfsf10 Kit ELISA
- Sujet
- TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. It is encoded by the TNFSF10 gene, which maps to 3q26, spans approximately 20 kb and contains 5 exons. TRAIL shows homology to other members of the tumor necrosis factor superfamily. It is composed of 281 amino acids and has characteristics of a type II transmembrane protein. TRAIL might induce apoptosis of brain tissue, indicating a potential target for treatment of multiple sclerosis. In cells expressing DcR2, TRAIL binding therefore activates NFkappaB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.
- Pathways
- Apoptose, Positive Regulation of Endopeptidase Activity
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