IL-1 beta Kit ELISA

N° du produit ABIN2690985

Détail du produit IL-1 beta Kit ELISA

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Marque: BD OptEIA™

Analytical Method: Quantitative

Attributs du produit: The BD OptEIA™ Set contains the components necessary to develop enzyme - linked immunosorbent assays (ELISA). Sufficient materials are provided to yield approximately 20 plates of 96 wells if the recommended storage, materials, buffer preparation, and assay procedure are followed.

Ingrédients: Capture Antibody
Detection Antibody
Enzyme Reagent
Standards
Certificate of Analysis

Matériel non inclus: 1. 96-well Nunc-Immuno™ polystyrene Maxisorp ELISA flat bottom plates (ThermoFisher Scientific Cat. No. 442404) are recommended
2. Microplate reader capable of measuring absorbance at 450 nm
3. Precision pipettes
4. Graduated cylinder, one liter
5. Deionized or distilled water
6. Wash bottle or automated washer
7. Log-log graph paper or automated data reduction
8. Tubes to prepare standard dilutions
9. Laboratory timer
10. Plate sealers or parafilm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

BD OptEIA™ Human IL-1β ELISA Set II - Reactivity Hu

Volume d'échantillon: 100 µL

Plaque: Without plate

Protocole: 1. Add 100 µL diluted Capture Ab to each well. Incubate overnight at 4°C.
2. Aspirate and wash 3 times.
3. Block plates: 200 µL Assay Diluent to each well. Incubate 1 hr RT
4. Aspirate and wash 3 times.
5. Add 100 µL standard or sample to each well. Incubate 2 hr RT.
6. Aspirate and wash 5 times.
7. Add 100 µL Working Detector (Detection Ab + SAv-HRP) to each well. Incubate 1 hr RT
8. Aspirate and wash 7 times (with 30 sec to 1 min soaks)
9. Add 100 µL Substrate Solution to each well. Incubate 30 min RT in dark
10. Add 50 µL Stop Solution to each well. Read at 450 nm within 30 min with l correction 570 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: IL-1beta (IL1B Produits)

Synonymes: IL-1 Kit ELISA, IL1-BETA Kit ELISA, IL1F2 Kit ELISA, IL-1BETA Kit ELISA, IL1beta Kit ELISA, il1-b Kit ELISA, zgc:111873 Kit ELISA, IL-1B Kit ELISA, IL-1beta Kit ELISA, Il-1b Kit ELISA, IL1B Kit ELISA, IL-1 beta Kit ELISA, IL-1b Kit ELISA, interleukin 1 beta Kit ELISA, interleukin 1, beta Kit ELISA, IL1B Kit ELISA, il1b Kit ELISA, Il1b Kit ELISA

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome