NGFB Kit ELISA

N° du produit ABIN2702851

Détail du produit NGFB Kit ELISA

Antigène: NGFB (Nerve Growth Factor beta (NGFB))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 40-10.000 pg/mL

Seuil minimal de détection: 40 pg/mL

Application: ELISA

Fonction: Mouse beta-NGF ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Mouse Beta-Nerve Growth Factor (bNGF)

Sensibilité: 40 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: NGFB (Nerve Growth Factor beta (NGFB))

Autre désignation: beta-NGF (NGFB Produits)

Synonymes: NGFB Kit ELISA, ngfb Kit ELISA, ns:zft386 Kit ELISA, xx:zft386 Kit ELISA, zgc:109730 Kit ELISA, hsan5 Kit ELISA, beta-ngf Kit ELISA, Ngfb Kit ELISA, Beta-NGF Kit ELISA, HSAN5 Kit ELISA, beta-NGF Kit ELISA, nerve growth factor Kit ELISA, nerve growth factor b (beta polypeptide) Kit ELISA, beta-Nerve growth factor Kit ELISA, nerve growth factor (beta polypeptide) Kit ELISA, NGF Kit ELISA, ngfb Kit ELISA, FPV072 Kit ELISA, FPV076 Kit ELISA, ngf Kit ELISA, Ngf Kit ELISA

Sujet: Gene Names: Ngf Ngfb
Protein names: Beta-nerve growth factor (Beta-NGF)

ID gène: 18049

UniProt: P01139

Pathways: Signalisation NF-kappaB, Signalisation RTK, Regulation of Cell Size