NGFB Kit ELISA
-
- Antigène Voir toutes NGFB Kits ELISA
- NGFB (Nerve Growth Factor beta (NGFB))
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 40-10.000 pg/mL
- Seuil minimal de détection
- 40 pg/mL
- Application
- ELISA
- Fonction
- Mouse beta-NGF ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Mouse Beta-Nerve Growth Factor (bNGF)
- Sensibilité
- 40 pg/mL
- Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling NGFB Kit ELISA
- Top Product
- Discover our top product NGFB Kit ELISA
-
-
- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
-
- Antigène Voir toutes NGFB Kits ELISA
- NGFB (Nerve Growth Factor beta (NGFB))
- Autre désignation
- beta-NGF (NGFB Produits)
- Synonymes
- NGFB Kit ELISA, ngfb Kit ELISA, ns:zft386 Kit ELISA, xx:zft386 Kit ELISA, zgc:109730 Kit ELISA, hsan5 Kit ELISA, beta-ngf Kit ELISA, Ngfb Kit ELISA, Beta-NGF Kit ELISA, HSAN5 Kit ELISA, beta-NGF Kit ELISA, nerve growth factor Kit ELISA, nerve growth factor b (beta polypeptide) Kit ELISA, beta-Nerve growth factor Kit ELISA, nerve growth factor (beta polypeptide) Kit ELISA, NGF Kit ELISA, ngfb Kit ELISA, FPV072 Kit ELISA, FPV076 Kit ELISA, ngf Kit ELISA, Ngf Kit ELISA
- Sujet
-
Gene Names: Ngf Ngfb
Protein names: Beta-nerve growth factor (Beta-NGF)
- ID gène
- 18049
- UniProt
- P01139
- Pathways
- Signalisation NF-kappaB, Signalisation RTK, Regulation of Cell Size
-