Syndecan 1 Kit ELISA

N° du produit ABIN2703471

Détail du produit Syndecan 1 Kit ELISA

Antigène: Syndecan 1 (SDC1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 14-4000 pg/mL

Seuil minimal de détection: 14 pg/mL

Application: ELISA

Fonction: Human Syndecan-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human Syndecan-1. Other species not determined.

Sensibilité: 14 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples8 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Syndecan 1 (SDC1)

Autre désignation: Syndecan 1 (SDC1 Produits)

Synonymes: CG10497 Kit ELISA, CT27296 Kit ELISA, Dmel\\CG10497 Kit ELISA, Dsyn Kit ELISA, Syd Kit ELISA, Syn Kit ELISA, dSdc Kit ELISA, l(2)10608 Kit ELISA, sdc Kit ELISA, syd Kit ELISA, syn Kit ELISA, Syn1 Kit ELISA, cd138 Kit ELISA, synd1 Kit ELISA, xSyn1 Kit ELISA, syndecan1 Kit ELISA, Syndecan-1 Kit ELISA, XSyndecan-1 Kit ELISA, SDC1 Kit ELISA, CD138 Kit ELISA, SDC Kit ELISA, SYND1 Kit ELISA, syndecan Kit ELISA, AA408134 Kit ELISA, AA409076 Kit ELISA, Sstn Kit ELISA, Synd Kit ELISA, Synd1 Kit ELISA, syn-1 Kit ELISA, HSPG Kit ELISA, SYNDECA Kit ELISA, Syndecan Kit ELISA, Syndecan Kit ELISA, syndecan 1 Kit ELISA, Sdc Kit ELISA, sdc1 Kit ELISA, SDC1 Kit ELISA, Sdc1 Kit ELISA

Sujet: Gene Names: SDC1 SDC
Protein names: Syndecan-1 (SYND1) (CD antigen CD138)

ID gène: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism