ZAP70 Kit ELISA

N° du produit ABIN2747924

Détail du produit ZAP70 Kit ELISA

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Épitope: pTyr493, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit. This assay semi-quantitatively measures phophorylated ZAP70 (Tyr493) and Total ZAP70 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human ZAP70 phosphorylated at site Tyrosine-493 and Total ZAP70.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Autre désignation: ZAP70 (ZAP70 Produits)

Synonymes: SRK Kit ELISA, STCD Kit ELISA, STD Kit ELISA, TZK Kit ELISA, ZAP-70 Kit ELISA, 70kDa Kit ELISA, AI327364 Kit ELISA, Srk Kit ELISA, mrtle Kit ELISA, mur Kit ELISA, zap70 Kit ELISA, MGC80670 Kit ELISA, MGC88962 Kit ELISA, ZAP70 Kit ELISA, zgc:110383 Kit ELISA, zeta chain of T-cell receptor associated protein kinase 70 Kit ELISA, putative tyrosine-protein kinase zap-70 Kit ELISA, zeta-chain (TCR) associated protein kinase Kit ELISA, zeta chain of T cell receptor associated protein kinase 70 Kit ELISA, zeta-chain (TCR) associated protein kinase 70kDa Kit ELISA, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog Kit ELISA, zeta chain of T cell receptor associated protein kinase 70kDa Kit ELISA, tyrosine-protein kinase ZAP-70 Kit ELISA, ZAP70 Kit ELISA, Smp_149470 Kit ELISA, Zap70 Kit ELISA, zap70.L Kit ELISA, zap70 Kit ELISA, LOC707555 Kit ELISA, LOC100342021 Kit ELISA, LOC100390922 Kit ELISA, LOC100542877 Kit ELISA

Sujet: Zeta-chain associated protein kinase 70 (ZAP70) phosphorylated at Tyrosine-493 and Total ZAP70

ID gène: 22637

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway