NFKBIA Kit ELISA
-
- Antigène Voir toutes NFKBIA Kits ELISA
- NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
- Épitope
- pSer32
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human Phospho-I-kappa B alpha (S32) ELISA Kit. This assay semi-quantitatively measures phophorylated I-kappa B alpha (Ser32) in lysate samples.
- Type d'échantillon
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Human IkB-alpha phosphorylated at site Serine-32.
- Attributs du produit
-
- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Matériel non inclus
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling NFKBIA Kit ELISA
- Top Product
- Discover our top product NFKBIA Kit ELISA
-
-
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Procédure de l'essai
-
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Date de péremption
- 6 months
-
- Antigène Voir toutes NFKBIA Kits ELISA
- NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
- Autre désignation
- IKBa (NFKBIA Produits)
- Synonymes
- IKBA Kit ELISA, MAD-3 Kit ELISA, NFKBI Kit ELISA, AI462015 Kit ELISA, Nfkbi Kit ELISA, RL/IF-1 Kit ELISA, ECI-6/IKBA Kit ELISA, NFKBIA Kit ELISA, ikbaB Kit ELISA, wu:fb81f08 Kit ELISA, zgc:56666 Kit ELISA, zgc:77616 Kit ELISA, ikbaA Kit ELISA, wu:fj16a07 Kit ELISA, zgc:85769 Kit ELISA, NFKB inhibitor alpha Kit ELISA, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha Kit ELISA, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b Kit ELISA, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a Kit ELISA, NFKBIA Kit ELISA, Nfkbia Kit ELISA, nfkbiab Kit ELISA, nfkbiaa Kit ELISA
- Sujet
- NF-kappa-B inhibitor alpha (IκB-alpha) phosphorylated at Serine-32
- ID gène
- 4792
- UniProt
- P25963
- Pathways
- Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling
-