NFKBIA Kit ELISA

N° du produit ABIN6244346

Détail du produit NFKBIA Kit ELISA

Antigène: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human IkB-alpha/NF-kappa-BIA ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human IkB-alpha.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Autre désignation: IkB-alpha (NF-kappa-BIA) (NFKBIA Produits)

Synonymes: IKBA Kit ELISA, MAD-3 Kit ELISA, NFKBI Kit ELISA, AI462015 Kit ELISA, Nfkbi Kit ELISA, RL/IF-1 Kit ELISA, ECI-6/IKBA Kit ELISA, NFKBIA Kit ELISA, ikbaB Kit ELISA, wu:fb81f08 Kit ELISA, zgc:56666 Kit ELISA, zgc:77616 Kit ELISA, ikbaA Kit ELISA, wu:fj16a07 Kit ELISA, zgc:85769 Kit ELISA, NFKB inhibitor alpha Kit ELISA, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha Kit ELISA, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b Kit ELISA, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a Kit ELISA, NFKBIA Kit ELISA, Nfkbia Kit ELISA, nfkbiab Kit ELISA, nfkbiaa Kit ELISA

ID gène: 4792

UniProt: P25963

Pathways: Signalisation NF-kappaB, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling