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JNK Kit ELISA

MAPK8 Reactivité: Humain, Souris, Rat pThr183 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2748302
  • Antigène Voir toutes JNK (MAPK8) Kits ELISA
    JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
    Épitope
    pThr183, pTyr185
    Reactivité
    Humain, Souris, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA Kit. This assay semi-quantitatively measures phophorylated JNK (Thr183/Tyr185) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human/Mouse/Rat Phospho-JNK (pThr183/Tyr185).
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Bizargity, Liu, Wang, Hancock, Visner: "Inhibitory effects of pirfenidone on dendritic cells and lung allograft rejection." dans: Transplantation, Vol. 94, Issue 2, pp. 114-22, (2012) (PubMed).

    Stephens, Stephens, Hobbs, Hutmacher, Bacic-Welsh, Woodruff, Morrison: "Myocyte enhancer factor 2c, an osteoblast transcription factor identified by dimethyl sulfoxide (DMSO)-enhanced mineralization." dans: The Journal of biological chemistry, Vol. 286, Issue 34, pp. 30071-86, (2011) (PubMed).

  • Antigène Voir toutes JNK (MAPK8) Kits ELISA
    JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
    Autre désignation
    JNK (MAPK8 Produits)
    Synonymes
    JNK Kit ELISA, JNK-46 Kit ELISA, JNK1 Kit ELISA, JNK1A2 Kit ELISA, JNK21B1/2 Kit ELISA, PRKM8 Kit ELISA, SAPK1 Kit ELISA, SAPK1c Kit ELISA, AI849689 Kit ELISA, Prkm8 Kit ELISA, BSK Kit ELISA, BSK/DJNK Kit ELISA, Bsk Kit ELISA, CG5680 Kit ELISA, D-JNK Kit ELISA, D-junk Kit ELISA, DBSK/JNK Kit ELISA, DJNK Kit ELISA, DJNK/bsk Kit ELISA, Dmel\\CG5680 Kit ELISA, JNK/SAPK Kit ELISA, Jnk Kit ELISA, Junk Kit ELISA, SAPKa Kit ELISA, c-Jun Kit ELISA, dJNK Kit ELISA, jnk Kit ELISA, T10F20.15 Kit ELISA, jnk1 Kit ELISA, sapk1 Kit ELISA, mapk8 Kit ELISA, zgc:112379 Kit ELISA, JNKb Kit ELISA, mapk8b Kit ELISA, mitogen-activated protein kinase 8 Kit ELISA, Stress-activated protein kinase jnk-1 Kit ELISA, basket Kit ELISA, Protein kinase superfamily protein Kit ELISA, mitogen-activated protein kinase 8 L homeolog Kit ELISA, mitogen-activated protein kinase 8b Kit ELISA, mitogen-activated protein kinase 8B-like Kit ELISA, MAPK8 Kit ELISA, jnk-1 Kit ELISA, Mapk8 Kit ELISA, bsk Kit ELISA, ATMPK8 Kit ELISA, mapk8.L Kit ELISA, mapk8b Kit ELISA, LOC109094263 Kit ELISA
    Sujet
    C-Jun N-terminal kinase (JNK) phosphorylated at T183 and/or Y185
    ID gène
    5599
    UniProt
    P45983
    Pathways
    Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
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